Anusmita Paul

2 Questions 4 Answers 0 Followers

Questions related from Anusmita Paul

What should be the pH of my lysis if I want to express a 6xHis tagged protein with a pI 9.2 from BL21DE3?

My protein's pI lies at around 9.2. I'm trying to purify the protein from BL21DE3. What should be the pH of my lysis and elution buffer for a good amount of protein isolation?

28 May 2025 1,944 2 View

Why are the DNA protein complex stuck near the well in my EMSA native page?

I’m facing the issue where my DNA protein is stuck near the wells in case of my EMSA native page. My protein’s theoretical pI is estimated to be 9.3. The pH of my 5x TBE running buffer is at...

05 May 2025 4,143 5 View