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Questions related from Anushka Upadhyay
If anybody has successfully done electroporation of RNA into HEK293T cell, kindly share the electroporation parameters. Or, if anyone is using Maxcyte STx machine for electroporation, please share...
29 January 2024 7,858 0 View
I have isolated plasmid and then linearised it with re. Then I put in-vitro transcription of the linearised plasmid but not band of mRNA could be observed only the linearised plasmid was seen....
20 November 2023 3,552 7 View
What size of plasmid can be isolated from qiagen miniprep and midiprep kit, respectively? And at what temperature shall I grow a 36kb plasmid with 12.5ug/ml chloramphenicol resistance?
08 September 2023 2,558 3 View
I am trying to isolate BAC plasmid of size 36kb. I tried with Qiagen Miniprep kit and Zymo BAC purification kit and ran on 0.5% gel. The bands can be seen on the gel but no bands were after I did...
01 September 2023 2,892 4 View
