Anurupa Ghosh

4 Questions 5 Answers 0 Followers

Questions related from Anurupa Ghosh

Anyone has a good protocol for Genotyping Mice using Tail snips?

Hi, Need help finding a good protocol for genotyping mice from tail snips. I really need suggestions on how to extract the DNA and have a good sample solution. My DNA in the sample solutions are...

05 August 2019 7,747 3 View

How to perform Thioflavin T assay for in vitro analysis of A-syn PFF's?

Hi, Does anyone know a good protocol to perform a Thioflavin T assay? I need to determine the extent of protein aggregation for my A-syn Fibrils which have been converted from A-syn Monomers.

01 August 2019 2,820 3 View

Hi, does anyone know a good cell viability assay to assess how toxic are alpha-syn fibrils?

We are currently trying to inoculate A-syn fibrils in the mouse striatum but before that we want to know how toxic these fibrils are by doing an in - vitro study. I was thinking MTT Assay is a...

29 July 2019 3,572 3 View

Primer needed to do genotyping for Line-61 (A53T) hemizygous mice?

Hi! My lab has acquired some line-61 mice from UCSD which are A53T mutant mice with Thy-1 promoter. I wanted to know how I can find a suitable primer for genotyping the pups from the mice . We...

14 July 2019 3,551 0 View