Can you please suggest a good working protocol for GUS assay in xylem vasculature of tomato root or for any other solanaceous crop?

I am not observing uniform induction of my promoter fused to GUS in xylem vasculature of taproot of tomato. Key questions are whether I should do cross-sections after or before putting in X-gluc...

15 August 2020 9,658 4 View