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Hi, I'm extracting bacterial DNA from CTAB/NaCl method. Starting material is either colonies or culture grown in LB broth. I follow the standard method, heat kill, lysozyme+TE 37C 3hrs, then...
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I expect to extract bacterial DNA in sputum/ bronchial washings of patients. I need to have at least 20ng/ul. manual methods are preferred.
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is it possible to infect cancer cell lines with bacterial infections such as tuberculosis?
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