Anum Safdar

3 Questions 4 Answers 0 Followers

Questions related from Anum Safdar

Why there is glow in the well after nested PCR?

Dear Researcher, I have used nested PCR to increase the specificity of Tetra Arms. Previously, I obtained results for another SNP using the same reaction conditions, and separate reactions...

15 July 2024 380 4 View

Why band for normal allele appears in a sample that is confirmed homozygous mutant?

I use confirmed homozygous mutant and homozygous normal samples for tetraARMS PCR optimization. everytime I run amplified products on gel I observe three bands instead of two bands. DNA samples...

28 June 2024 8,255 3 View

How can I recover the DNA from the column?

After completing DNA extraction from whole blood using the QIAamp DNA Mini kit but obtaining no yield, I suspect the DNA might be retained in the column. How can I recover the DNA from the column.

27 March 2024 9,086 2 View