Anu Jaglan

4 Questions 1 Answers 0 Followers

Questions related from Anu Jaglan

Why GST-tagged recombinant protein containing BL21 cell become viscous after three cycle of freeze thaw at -80 degree Celsius?

i was cloned the lysin gene of bacteriophage in the pGEX 4t1 vector and transformed them in the BL21 Competent cell. competent cell was transformed and upon induction they are showing the...

04 July 2023 6,857 1 View

How to change the elution buffer with tris Cl buffer before cleavage of thioredoxin tag from recombinant protein with enterokinase?

my recombinant vector contain thioredoxin tag and my elution buffer contain imidazole, NaCl and NaH2PO4. so how can i replace my elution buffer with tris-Cl buffer before enteokinase treatment

07 June 2023 4,554 4 View

Why the E.coli cell containing the recombinant cell does not express that particular protein upon induction with iptg?

i am cloning my gene of interest in vector pet32 and pgex4t. upon transformation i got colony on antibiotic containing LB Agar. also colony pcr and pcr with the plasmid extracted from the clone...

23 April 2023 1,125 4 View

Is freeze thaw is sufficient to lyse the e.coli BLA21 Cell containing recombinant protein?

While performing sonication i am facing the issue of frothing which i think results in the aggregation of desired protein and leads to the formation of inclusion bodies

26 February 2023 830 2 View