4 Questions 6 Answers 0 Followers
Questions related from Anu C J
Hi, I am going to do Percoll gradient centrifugation for subcellular fractionation. Before I start I would like to know whether I need to adjust the PH of percoll to 7.4. I use percoll from...
20 November 2018 2,522 3 View
I am trying to identify a protein with very low expression which is labelled with H2B-GFP. I tried to identify GFP expression using anti GFP antibodies. however, no bands observed in western blot...
25 April 2018 8,202 4 View
Is immunofluorescence staining or western blot is more sensitive to study the protein expression?
26 March 2018 5,979 8 View
Dear all, Recently I started to work with immunofluorescence analysis. I want to know the method of mounting slides after staining procedure. I used Everbrite hardset mounting medium after...
17 March 2018 6,737 10 View
