Antony Philip Jackson

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Questions related from Antony Philip Jackson

How quantitative is the in-situ proximity-ligation assay?

Hi, We have been carrying out an in situ proximity ligation assay (PLA) to detect the close association of two proteins on the plasma membrane of HEK293 cells. We find that the PLA signal...

06 December 2018 6,752 0 View

Hi, How easy is to detect disulphide bonds and/or free cysteines in a protein using CD spectroscopy?

We have a fairly small (10 kDa) protein that shows some evidence of secondary structure by CD. It has four cysteines and we expect these to form two disulphide bonds. But reduction under...

29 July 2017 6,524 0 View