Antonios Chionis

2 Questions 5 Answers 0 Followers

Questions related from Antonios Chionis

Best RT enzyme and best PCR enzyme for high GC content (>65%) RT-PCR?

I have thus used superscript II and IV :/ I denature RNA at 65 degrees, I wonder if I can increase that to a temperature at which DNA is usually denatured at (around 94 degrees) without...

09 September 2016 3,415 6 View

Should I use isolated RNA or purified mRNA for RT-PCR?

Will isolate RNA from tissue. I will not be running RT-qPCR, but Reverse Transcription PCR. For qPCR typically RNA is used instead of mRNA, I wonder if the same applies to RT-PCR. I only want to...

05 May 2016 933 7 View