Antonios Chionis

3 Questions 4 Answers 0 Followers

Questions related from Antonios Chionis

Best way to extract RNA from FFPE human brain tissue?

I am trying to compare yield of fresh vs FFPE and the measure relative quantity by RT-qPCR of housekeeping gene. I can upload the protocol I am using right now if anyone is interested in sharing...

12 December 2016 861 2 View

Best RT enzyme and best PCR enzyme for high GC content (>65%) RT-PCR?

I have thus used superscript II and IV :/ I denature RNA at 65 degrees, I wonder if I can increase that to a temperature at which DNA is usually denatured at (around 94 degrees) without...

01 September 2016 6,183 5 View

Should I use isolated RNA or purified mRNA for RT-PCR?

Will isolate RNA from tissue. I will not be running RT-qPCR, but Reverse Transcription PCR. For qPCR typically RNA is used instead of mRNA, I wonder if the same applies to RT-PCR. I only want to...

16 May 2016 4,001 6 View