Antonio di stasi

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Questions related from Antonio di stasi

How do you clone 1 or 2 inserts with cloning ends using the same enzyme?

What are your optimization strategies when cloning with a single enzyme at both ends? What if you have 2 inserts? Did you ever try to join the insert and a PCR product using chimeric internal...

01 January 2015 3,687 5 View

Has anyone compared infusion vs. restriction enzyme based cloning?

Which one is more efficient? When can infusion be more indicated?

01 January 2015 873 5 View

How do you prepare your PCR for cloning?

How long do you digest your PCR? Do you believe cloning efficiency improves when isolating the band on a gel, rather then simply purifying it?

01 January 2015 7,971 8 View