Antonio Marino

2 Questions 5 Answers 0 Followers

Questions related from Antonio Marino

Could the cloning have affected my plasmid copy number?

Hi everyone, Here it is the problem I'm dealing with at the moment: I have successfully cloned my insert of interest (4.2 kb.) into my backbone plasmid (4.3 kb.). Before that I had no problem...

24 February 2019 9,936 6 View

Cloning strategy... Am I doing right?

Dear community I'm having issues during my cloning. I have to clone an insert of 4.2kb in a plasmid of 4.3 kb. During my first try i opened the plasmid using EcoRV a than I used CIP enzyme to...

07 June 2018 2,342 5 View