Antonia Aste

2 Questions 1 Answers 0 Followers

Questions related from Antonia Aste

At what temperature (30 or 37ºC) should I prepare shuffle E. coli chemocompetent cells?

We have recently purchased competent shuffle E. coli cells from NEB, and we have to prepare more chemically competent cells, but I don't know if it would be better to grow the starter culture and...

20 December 2023 5,700 1 View

Can the ligation buffer decrease the efficiency in e. coli TOP10 electroporation?

I am having some trouble with the transformation protocol of electrocompetent TOP10 E. coli cells. At first I thought it could be because of the size of the plasmid (10 kb) so I electroporated...

26 December 2022 6,964 5 View