Antonella Sialer

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Questions related from Antonella Sialer

RT Negative Control amplifying same as my cDNA samples (same transcript size)?

Hi to everyone! I have been having an issue with my retrotranscription negative controls (I have 2 samples and it happens in both). I am using a GAPDH primer (F & R - 500 nM) for...

16 September 2024 7,631 5 View

Why does the Nancy-520 dye for electrophoresis solidify or crystallize?

Hi, I have had some issues with the Nancy-520 dye (it has expired but it had not been open), which I see it got like a crystall once opened (it was stored in 4 ºC). I could see that if it was...

05 August 2024 7,361 1 View