Anton Govednik

3 Questions 8 Answers 0 Followers

Questions related from Anton Govednik

Which primers to use for studying soil 16S (archaeal and bacterial) community and ITS (fungal) community?

Hi, is there any general concensus on which would be the best 16S rRNA primers to use to cover both bacterial and archaela community? What about fungal ITS? In the literature I've came across...

01 December 2022 5,463 0 View

How to decide on when to use T4 gp32, BSA and DMSO use in qPCR for quantifying soil microbial community?

Dear all, I hope you could help me with the following question: There are different adjuvants that are used in qPCR for quantifying soil microbial functional genes (which often means using...

02 March 2022 9,945 0 View

Smearing of qPCR products on a gel after a single melt curve peak?

Hello everyone, I have the following problem: I'm observing a lot of smear after loading qPCR products on 1.3% gel (attached pUC19-M13_primers_test) eventhough the melt curves seem nice with only...

25 February 2022 4,553 6 View