Anton Bilsen

2 Questions 1 Answers 0 Followers

Questions related from Anton Bilsen

Why does transient transfection with a linear plasmid result in higher gene expression than with a circular plasmid?

Hi everyone, I'm trying to get Sf9 (insect) cells to express a baculoviral gene (vp80) through a plasmid-based expression system. I used qPCR to compare vp80 transcript levels among cells...

22 April 2025 4,936 3 View

Does azido labelling have any advantages over antibodies when looking for presence/absence of certain glycan-linked molecules?

Hi everyone, Suppose I want to look for glycan-bound (specifically O-acetylglucosamine-bound) molecules, such as certain proteins or lipids, in a cell sample. In this scenario, I am not interested...

17 May 2022 6,032 0 View