Antoine Stier

3 Questions 10 Answers 0 Followers

Questions related from Antoine Stier

What is the best method to quantify ROS production in (fresh) tissue homogenate?

I wish to measure ROS production in fresh tissue homogenate (brain, liver, heart), but I am struggling to find a proper method to do that. AmplexRed does not seem ideal in this case because of the...

02 March 2016 770 11 View

Is it possible to measure enzymatic activities of mitochondrial ETC complexes from cells frozen at -20°C?

Dear RG community, I wish to measure enzymatic activity of mitochondrial electron transport chain components (complex II, III and IV) using frozen blood cells. However, I will only have access to...

10 July 2015 5,011 10 View

Is there interference between haemoglobin and oxidative stress measurements (total antioxidant capacity/oxidative damage such as lipid peroxidation)?

To compare oxidative stress in erythrocytes between mammals and birds, I need to know if haemoglobin is an interfering agent. Some papers gave this information while other don't.

27 September 2012 3,191 8 View