GFP-tubulin in HeLa — why just cytosolic?

Want to visualize microtubule dynamics in live cells. Transfecting HeLa cells with GFP-Tubulin (Addgene) using Jet PEI (4h transfection — image 24h later). Problem: GFP looks cytosolic without a...

23 October 2013 8,249 18 View