Antara Chakravarty

3 Questions 5 Answers 0 Followers

Questions related from Antara Chakravarty

How to correctly take UV-vis spectra of POPC liposomes and interpret the results?

Hello everyone, I am new to liposome characterization and hope to get your insights about an experiment I am running. I took UV-vis spectra for POPC liposomes (undiluted and a set of dilutions...

31 August 2022 7,645 0 View

How to label POPC liposomes with DPH to measure fluidity using fluorescence polarization?

Hi everyone, I have been using this technical note from BMG Labtech (https://www.bmglabtech.com/en/application-notes/membrane-fluidity-measurement-using-uv-fluorescence-polarization/) and trying...

09 August 2022 3,460 2 View

How to calculate initial velocity of an enzyme from RFU vs time graph?

Hi everyone, I have been trying to calculate the Km value of a recombinant HCV NS3/4A protease using a FRET substrate, the cleavage of which can be detected at 500 nm using excitation wavelength...

23 December 2021 4,711 14 View