Anshuli Razdan

4 Questions 8 Answers 0 Followers

Questions related from Anshuli Razdan

Can a human cancer cell line have heterogenous population having different chromosomal number, which can be sorted by flow cytometry?

Hi, I have been doing flow cytometry for cell cycle analysis using PI for a liver cancer cell line and I have been getting a big and strange peak where S-phase should be. Not sure if it's S-phase...

30 April 2019 733 7 View

Better control for CCA/ apoptosis in flow cytometry?

Hi, I am a beginner at flow cytometry and I am interested in investigating the effects of various drugs/inhibitors on cell proliferation/growth and apoptosis. Before jumping to flow, I have done...

06 September 2018 8,501 0 View

Does use of antibiotics (Pen/strep) in cell culture media affect IC50 of drugs in cell viability assay (MTS)?

I generally use antibiotics for routine culture of cells but don't use it while doing MTS assay. I am not sure if use of antibiotics should give me aberrant IC50 value of drugs? Any...

27 April 2018 6,958 5 View

No Insert/ Vector only colonies?

Hi, I am new at cloning, and having troubles in finding the desired clone. After transformation, I am getting the colonies containing only the vector. This time, my vector only control had 1...

06 February 2017 1,997 9 View