Anshul I Arora

2 Questions 6 Answers 0 Followers

Questions related from Anshul I Arora

Anyone experience in western blot troubleshooting?

I am trying to do a western blot and the protein transfer is confirmed by Ponceau stain. However, I never get bands on the x-ray film. I am new to the lab, so I started with b-actin...

27 March 2018 3,373 10 View

Does anyone has experience with troubleshooting inconsistent ELISA results?

I am trying to do indirect ELISA from human serum samples. The protein mixture we use as antigen is made in our laboratory. I coat with same concentration and in multiple dilutions. The serum...

07 February 2018 4,740 1 View