Anny Velasquez Cruz

2 Questions 1 Answers 0 Followers

Questions related from Anny Velasquez Cruz

It is more frequent to find amplification errors closer to primers (say, 1-10 nt away from primer) or this just happen at random?

I'm looking for SNP variation in a population and in an article I found that the primer they use is really close (3nt) away to the SNP target, so I was wondering if there's a possibility that...

02 February 2020 4,934 3 View

The same conditions for conventional PCR could be used for qPCR?

I have a protocol for conventional PCR, the amplicon size is around 100bp and the number of cycles between 25-30. I supposed the temperature and time of each phase must be the same because I used...

12 December 2018 2,222 1 View