I am seeing 45 kDa protein in SDS PAGE gel instead of 16 kDa protein. Why?

I expect a 16 kDa recombinant protein to be expressed in E.coli. However, I could see 45 kDa protein in the SDS PAGE gel after induction. My protein has (His)6 tag, but no bands seen at 16 kDa and...

02 January 2025 2,089 4 View