Annia Gonzalez-Hernandez

2 Questions 8 Answers 0 Followers

Questions related from Annia Gonzalez-Hernandez

How can we separated the fragments generated by pepsin digestion of antibodies?

We try in the lab a method for digestion of whole IgG subclass with pepsin to separated Fc and Fab fragments. But we have problems with desalting of fragments. this step it is very important to to...

04 April 2016 6,526 4 View

Someone has the optimun conditions for the papain digestions of antibodies?

My student and I tray to obtains Fc and Fab fragments from whole human IgG subclass whit this experimental conditions: ratio papain / Ab (3% ) (w/w), reaction time (24h), final volume (150µL) and...

04 April 2016 4,366 5 View