Anne-Sophie Bouchon

4 Questions 4 Answers 0 Followers

Questions related from Anne-Sophie Bouchon

Does buffer molarity impact fungus reisolation from plant samples?

Hi, I am conducting an experiment for reisolate Verticillium dahliae from cacao. I am thinking to grind my sample in phosphate buffer and PVP (15 g/l). I am adding PVP because my samples are quite...

22 August 2019 646 3 View

How to prevent degradation of –(-)Epicatechin from cacao in the lab?

Hi, I want to measure –(-)Epicatechin from cacao by fluorimetry. I have used acetone, water, acetic acid (70%, 29.5%, 0.5%) and sonication for the extraction. I have to let the sample evaporate...

15 August 2019 814 4 View

What is the best solvent for spore germination test with sulphur?

Hi, I would like to dissolve sulfur (50µg/ml) to assess the germination of conidia from Verticillium dahliae with it. So, I need a solvent that is safe and do not affect the germination of spores...

03 August 2019 2,358 2 View

How to remove PVP into enzymatic extracts

Hi, I have extracted enzyme (PPO) in phosphate buffer and PVP (15g/L) and then centrifuge (12,000 g, 10 min). This seems this concentration seem to inhibit the PPO. So, I want to remove it to...

01 January 1970 7,323 5 View