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Questions related from Anne-Sophie Bouchon
I've read that half of the organic matter in compost consists of carbon. However, I'm not sure if it's appropriate to use this conversion factor for all phases of composting when producing button...
21 April 2023 6,896 2 View
Hi, I have used fluorimetry at excitation : 280nm and emission 320 nm with cacao leaf or petiole samples. I would like to know what compounds are measured except epicatechin and catechin monomers...
19 March 2020 1,021 0 View
Hi, I am conducting an experiment for reisolate Verticillium dahliae from cacao. I am thinking to grind my sample in phosphate buffer and PVP (15 g/l). I am adding PVP because my samples are quite...
22 August 2019 657 3 View
Hi, I want to measure –(-)Epicatechin from cacao by fluorimetry. I have used acetone, water, acetic acid (70%, 29.5%, 0.5%) and sonication for the extraction. I have to let the sample evaporate...
15 August 2019 826 4 View
Hi, I would like to dissolve sulfur (50µg/ml) to assess the germination of conidia from Verticillium dahliae with it. So, I need a solvent that is safe and do not affect the germination of spores...
03 August 2019 2,369 2 View
Hi, I want to count Verticillium dahliae CFU per mg of fresh plant material. I was thinking first simply to ground the tissue and let it macerate shortly in phosphate buffer. But, the problem to...
20 June 2019 8,224 0 View
Hi, I have extracted enzyme (PPO) in phosphate buffer and PVP (15g/L) and then centrifuge (12,000 g, 10 min). This seems this concentration seem to inhibit the PPO. So, I want to remove it to...
01 January 1970 7,335 5 View
