Anne Barry-Reidy

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Questions related from Anne Barry-Reidy

How can I eliminate non-specific amplification during SYBR qPCR?

I have been designing qPCR primers for use in assays with Promega GoTaq qPCR mastermix. This uses BRYT Green, which is supposed to be comparable to SYBR chemistry. Using a non-precious positive...

26 August 2015 1,666 8 View

How can I generate reliable qPCR efficiency curves for genes expressed at very low levels?

Hello all, I am trying to design qPCR assays for a family of inducible innate immune peptides which are expressed at very low levels basally. When running standard curves to calculate primer...

08 July 2015 8,916 9 View