Annalisa Varriale

4 Questions 12 Answers 0 Followers

Questions related from Annalisa Varriale

Can anyone offer advise regarding low A260/A230 and A260/A280 ratios?

I have extracted DNA (proteinase k, chloroform extraction followed by isopropanol and ethanol 70) from a biological fluid and checked its purity with nanodrop: unfortunately the values of these...

24 April 2014 892 3 View

Absence of contaminant nucleic acids when extracting DNA from plant leaves - any thoughts?

I am extracting DNA from leaves taken from a tree growing in the field. I was wondering how I can be sure that DNA extracted comes only from the plant and not from contaminating agents (mainly...

17 April 2014 3,254 3 View

How to obtain a very high amount of plant DNA?

Does anyone have an idea of how much plant tissue (Leaves grounded in liquid nitrogen) should be processed in order to get at least 1 gram of DNA by classical extraction methods (CTAB)?

11 April 2014 8,359 10 View

How to obtain a clear supernatant from plant leaves DNA extraction?

I tried the CTAB protocol and a salting out protocol for DNA extraction from mature leaves of a tree. The supernatant is in both cases dark green and I suppose that DNA solution still contains...

26 March 2014 5,892 6 View