Anna Oliveira

4 Questions 3 Answers 0 Followers

Questions related from Anna Oliveira

Gene ontology without global gene expression analysis?

Hi everyone! I've been study the expression of 5 microRNAs using TaqMan assays in several cell lines (microRNAs were selected through literature review) and I obtained statistically significant...

02 February 2020 7,591 3 View

How many ng of RNA should I use to make cDNA?

Do you think that 100ng of RNA is sufficient to obtain good Cts in a qPCR?

04 April 2018 2,473 10 View

Can i use the ΔCt method when the efficiency of my qPCR is under 90%?

I've been making qPCRs with SYBR Green and in some of my reactions the efficiency is under 90% (between 80%-89%) Can i use the ΔCt method to analyse my results? If not, what should i do?

04 April 2018 4,755 5 View

Concentration of RNA to make cDNA?

I have about 100 samples to study the expression of a gene by qPCR. (GADPH is the endogenous control) I have already extract the RNA from all the samples and i went to nanodrop to quantify them....

03 March 2018 3,955 8 View