Anna Mendaluk

5 Questions 10 Answers 0 Followers

Questions related from Anna Mendaluk

What differentiation marker do you use to prove differentiation of SHSY5Y (Western Blot)?

Hello! I am trying to differentiated SH SY5Y cells using all-trans Retinoic Acid. I kept the cells for 7 days with 10uM RA (in one experiment I had 3%FBS in medium, and in other 10%FBS). Cells...

02 February 2015 7,791 3 View

How do I prove differentiation of SH-SY5Y?

Hi! I am during differentiation for SH-SY5Y cells. How can I prove that they really undergo differentiation? I can see some morphology changes and some neurites outgrowth, but I need some stronger...

12 December 2014 3,470 7 View

Why does my HEK293 become round when they are growing on Poly-L-lysine coated coverslips?

Hi, I have noticed that my HEK293 cells become round shaped when I grow them on poly-L-lysine coated coverslips. They are not dead, I seeded the same amount of cells from the same tube on coated...

11 November 2014 4,715 4 View

What is the reason for problems with big errors after standardization of Western Blot results?

Hi! I am trying to check effect of degradation of some protein in cells after treatment with compounds. I performed few trials and I did standardization/normalization to loading control and to...

11 November 2014 2,567 14 View

Why did my Western Blot stop working?

Hello everyone!I was performing a WB for 3 months. First 2 months everything was ok and since 1 month I am not able to get good results. It looks like just random bands show up - at the correct...

10 October 2014 287 11 View