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Dear friends! I designed, checked, and ordered primers for RT PCR. How can I verify them as working now? 1. How to choose the temperature for best amplification (the primers Tm is 60,...
27 August 2021 6,654 7 View
Dear friends! Due to the project, we have 8 groups of rats (n=6), my task is to perform PCR. It includes the expression of 8 genes, 3 microRNA, 4 lncRNA. I`m a bit stressed about the quantity of...
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Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...
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