Anna Lesniak

3 Questions 2 Answers 0 Followers

Questions related from Anna Lesniak

How to identify a receptor based on G-protein activation?

Dear all, I'm currently working on establishing the mechanism behind a modulatory effect of a drug candidate on opioid analgesia. We believe that it does so by activating GDP/GTP exchange in the...

11 March 2023 4,887 1 View

How to determine the Ki value from the GTPgammaS assay?

Dear RG researchers, I have been wondering is there any way to calculate the Ki value of an agonist in the [35S]GTPgammaS assay? It only gives the EC50 value, but is it possible to obtain the Kd...

23 June 2020 7,771 0 View

Does anyone know how to deal with a weak agonist-stimulated [35S]GTPγS signal?

Hi everyone! I'm currently optimizing my [35S]GTPγS assay in whole rat brain homogenates and I'm only getting 50% stimulation over basal with 10uM DAMGO. Another thing is that the difference in...

25 December 2016 2,295 2 View