Anna Lesniak

4 Questions 4 Answers 0 Followers

Questions related from Anna Lesniak

How to identify a receptor based on G-protein activation?

Dear all, I'm currently working on establishing the mechanism behind a modulatory effect of a drug candidate on opioid analgesia. We believe that it does so by activating GDP/GTP exchange in the...

11 March 2023 4,955 1 View

How to determine the Ki value from the GTPgammaS assay?

Dear RG researchers, I have been wondering is there any way to calculate the Ki value of an agonist in the [35S]GTPgammaS assay? It only gives the EC50 value, but is it possible to obtain the Kd...

23 June 2020 7,841 0 View

Does anyone know how to deal with a weak agonist-stimulated [35S]GTPγS signal?

Hi everyone! I'm currently optimizing my [35S]GTPγS assay in whole rat brain homogenates and I'm only getting 50% stimulation over basal with 10uM DAMGO. Another thing is that the difference in...

25 December 2016 2,324 2 View

Why does radioligand binding does not reach equilibrium?

Hi everyone! I got a bit of an issue with my radioligand receptor binding. I've started with standard kinetic and saturation experiments to determine the incubation time and Kd. The thing is that...

28 January 2015 4,956 4 View