How can I obtain good RNA yield from freshly isolated PBMCs and less than 200 uL whole blood?

Hello, I have been trying to extract RNA from about 1.5x10^5 freshly isolated human PBMCs with the Qiagen Rneasy Mini kit. The fresh blood is layered on top of Cedarlane lympholyte in a falcon and...

06 December 2015 9,789 7 View