Anna garcia puig

6 Questions 7 Answers 0 Followers

Questions related from Anna garcia puig

How can I link a mir30-shRNA to a fluorecent protein?

I am generating a construct to make an animal line. I would like to express a marker before a mir30-based shRNA (CMV promoter - marker - mir30shRNA-SV40pA). How can I link this two elements so...

13 November 2015 8,536 5 View

How do I screen zebrafish embryo phenotypes?

Is there any checklist to screen zebrafish embryo in order to find malformations (eyes, tail...)? Which parameters do I have to take into account? I want to see the affectations of my embrios once...

12 November 2015 2,445 2 View

I am getting no signal with an ISH probe that worked previously. What can be happening?

I am doing In situ hybridizations on zebrafish cryosections (embeded in OCT) and I can't make them work. I use to follow the same protocol one year ago and it worked perfecly, but now I can't see...

30 September 2015 3,233 6 View

How do I check the efficiency of a pair of qPCR primers?

I am new at the qPCR field, and I would like to know how do I can check the efficiency of a pair of primers. I read that I have to make serial dilutions of a DNA (with known concentration), but I...

27 May 2015 2,162 9 View

Does anybody know how can I hoomogenyze a native heart ECM into a solution?

I obtained ECM from cardiac tissue (I don't have much sample) and I want to make a solution of this ECM to be able to make a coating for cellular culture. Does anybody know how can I homogenize...

16 April 2015 588 5 View

Can a solution of Pepsin in 0,1M HCl be stored at -20ºC, or will it lose its activity?

I prepared a solution of Pesin in 0,1M HCl and I stored it at -20ºC. Due to the concentration of pepsin that I need in this solution, I need to prepare a lot of solution in comparison to the...

13 April 2015 6,907 4 View