LAMP experiment showing false positive, initially it worked for 3-4 experiments. what is the problem?

Hello everyone, I am having an issue with my LAMP reaction for target DNA detection (30ng). I was using the standard NEB protocol using BST 2.0 polymerase, with 1.6 uM FIP/BIP, 0.2 uM F3/B3 and...

07 February 2025 2,240 4 View