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Questions related from Ankita Gupta
We obtained M13 ssDNA from NEB and transformed suitable host bacterial strain followed by phage preparation from the same. Phage was then used for purification of ssDNA by PEG precipitation and...
09 September 2014 8,753 8 View
My objective is to purify protein from insect cells but I suspect a problem with the bacmid. Upon transfection of the cells with non-recombinant bacmid using cellfectin, cells do undergo...
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I have cloned a gene in a plasmid which is without a tag and I am going to use the baculovirus expression system for its expression and purification. Since there is no tag present with the gene...
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I have cloned a gene in pFastBac and confirmed its presence by restriction analysis. It was then transformed in DH10Bac cells carrying the bacmid. By site-specific transposition, the gene is to...
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