Why Ecoli cells (BL21 DE3 and Rosetta) aren't growing?

Hi, I have a peculiar problem which persists, so my strains BL21DE3 and Rosetta both have my GOI cloned in pET vectors with Ampicillin and Kanamycin resistance. I have grown them in shakeflasks...

15 March 2024 2,821 6 View