Ankita Kumari

3 Questions 4 Answers 0 Followers

Questions related from Ankita Kumari

No Ct value of genes was detected in H9c2 cells. The primers used were good with other rat cell lines and rat tissues. What could be the reason?

Hii I was amplifying some anti-oxidant and apoptosis genes with H9c2 cell line which is derived from rat myoblast. But surprisingly, I found No Ct value of these genes. Also, GAPDH and b-actin Ct...

27 March 2023 4,431 4 View

Does horse serum from HiMedia needs heat-inactivation for differentiation of H9c2 cells?

I am confused whether to heat inactivate the horse serum for differentiation of H9c2 cells or not. Thanks in advance. Ankita

28 November 2022 8,829 0 View

Smear in agarose gel of PCR product?

I was tried to get band for cloning of NLRP3 gene which is of 3000 bp length. For standardization of annealing temperature, I run gradient PCR with combination of different concentration of...

02 December 2021 9,906 3 View