Can anyone tell me if why i am getting my recombinant protein both in supernatant and pellet after lysis ?

hello all I am expressing a His6x recombinant protein and after lysis in lysis buffer i sonicate the cell suspension (40% amplitude 30 sec ON 30 sec OFF , 5 cycles). when i run SDS PAGE of the...

19 June 2020 5,625 12 View