Anju Pandey

3 Questions 4 Answers 0 Followers

Questions related from Anju Pandey

What wrong with our ligation?

We are trying to ligate FokI digested insert with BsaI digested and rSAP treated Vector. We got 5-10 colonies in LB+ selected antibiotic plates after heat-shock transformation as well. But when we...

29 October 2020 6,770 3 View

Is it necessary to do heat inactivation after ligation?

We are doing heat inactivation of ligation reaction at 65 C after 12 hours of incubation at 16 C but we are having a problem in getting colonies.

01 September 2020 8,104 3 View

How long we need to wash roots in PBS to reduce the auto fluorescence? Is there any protocol on it?

We have tried dipping roots for 10 min at PBS for three times. But we found that the roots were becoming translucent but they are still fluorescence. So seeking information regarding the time and...

01 September 2020 2,044 3 View