Hi! I am going to do a essential gene knockout (pBAD33) in Acinetobacter baumanii. Can you please suggest any good methods?

I have tried to do the gene knockout using the same plasmid in organisms like Vibrio cholera and Shigella flexnerii and failed. It will be very helpful if you can suggest a good protocol for...

11 November 2018 2,055 4 View