I have been isolating and cryopreserving human peripheral blood mononuclear cells for later flow cytometric analysis. What precautions should I take?

I have collected 10 ml blood in heparinized vacutainers. The pellet seems smaller than when I collect in EDTA ones. I cryopreserve in 10% DMSO, 40% human serum and 50% rpmi 1640 and then freeze...

04 April 2016 1,024 4 View