Anjali Anil

5 Questions 4 Answers 0 Followers

Questions related from Anjali Anil

Cloning and expression of 10 kDa protein in pET vector?

I have cloned a gene for a 10 kDa bacterial protein in pET28a (to be expressed as a C terminal His tagged protein) and transformed into DH5a. I have confirmed the clone by restriction digestion...

06 March 2018 6,681 4 View

5' Phosphate in Blunt End Ligation?

Consider that I cut my vector backbone with a blunt end cutter (EcoRV) and Iam not performing the alkaline phosphate treatment which will preserve the 5' phosphate on the vector. Is it necessary...

06 March 2018 4,930 3 View

Specific marker for Lysosomes?

Common lysosomal markers for immunofluorescence studies are LAMP1, CD63 and lysotracker which can also stain late endosomes. Can anyone suggest a marker/dye that is very specific for lysosomes?

04 February 2018 5,048 6 View

How does differentiated THP-1 macrophages look?

Iam using PMA at 25 ng/ml for 24h followed by washing and incubation for another 24h in fresh medium (RPMI+10%FBS) to differentiate THP-1 monocytes. Within this time duration, I definitely observe...

10 August 2016 5,590 4 View

Does the DNA always get integrated into the genome during stable transfections?

Few questions about stable transfections I have not been able to find proper answers to: 1. In a stable transfection, does the plasmid DNA always get integrated into the genome? In case the...

04 September 2015 7,460 10 View