Aniruddha Chatterjee

3 Questions 13 Answers 0 Followers

Questions related from Aniruddha Chatterjee

Quality of RNA for transcriptome study.

I have isolated RNA from Neutrophil. The RIN value is 7.1. It's quite tricky to isolate pure neutrophil from peripheral blood and then extract RNA from them. This is what I got. I want to do a...

12 December 2012 2,365 10 View

Can anyone commnet on biased aligners for bisulphite converted reads?

I mean due to their algorithm some aligners tend to align methylated sequences to the genome more efficiently or vice versa. Can anyone explain with some examples please.

12 December 2012 3,493 1 View

Searching SNPs in 40-220 bp length fragments

I have analysed my NGS data from Illumina (RRBS), I have fragments of information (chromosome, genomic position etc) and I would like to search against databases and see whether there are any...

07 July 2012 746 7 View