Anirban Manna

6 Questions 3 Answers 0 Followers

Questions related from Anirban Manna

Is the PDBsum webserver currently non-functional?

I modeled a protein using AF3 and uploaded the structure to the PDBsum webserver alongwith my email ID. But I have not received any email with the analyes of the uploaded structure. Are others...

24 November 2024 7,835 2 View

How to study protein-ligand interaction by protein intrinsic tryptophan fluorescence spectroscopy?

I am trying to study the interaction of my protein with a small molecule ligand by trp fluorescence spectroscopy. My protein has molecular weight of 16 kDa and has 2 tryptophan residues. I am...

26 March 2022 3,394 2 View

What is the minimum length of DNA probe for use in Electrophoretic Mobility Shift Assay (EMSA)?

I have used a 12 base pair DNA probe for EMSA, but the shifted bands that I am getting are very faint. Should I increase the length of the probe for getting a good shift? Please help.

19 December 2019 2,596 4 View

What percentage of glycerol should be used to preserve a purified protein (the protein is to be used for studies on protein-ligand interaction)?

The protein HSF1 (6X-His tagged at the N-terminus) was overexpressed in BL21(DE3) strain of E.coli and subsequently purified by Ni-NTA affinity chromatography. 300mM imidazole was used to elute...

28 February 2019 3,124 3 View

Which institutes in India can provide transgenic mice models for Amyotrophic Lateral Sclerosis, Parkinson Disease and Alzheimer Disease?

For elucidating the molecular mechanisms of these neurodegenerative diseases, study of transgenic mice models is a must.

15 December 2017 2,999 2 View

How do I get an even distribution of cells throughout the plate, while seeding cells in a 35 mm culture dish?

Growth medium added: 2 ml in each 35 mm plate 20 to 23 microlitre of cell suspension seeded in each plate

26 May 2016 3,293 10 View