Anil Panigrahi

4 Questions 106 Answers 0 Followers

Questions related from Anil Panigrahi

How long does it take to degrade target transcripts by siRNA?

Typically, the effect of siRNAs are assayed after 48hrs. During this period, the siRNA is expected to "keep degrading" the existing as well as newly synthesized transcripts. Now, if there is a...

12 December 2014 2,627 5 View

Do eRNAs remain attached with the template DNA or are detached and float around?

The last couple years have seen several reports of enhancer RNAs (eRNAs and similar transcripts) that aid enhancer- and activator-dependent transcription. Arguably, a chunk of 5' end of the eRNA...

08 August 2014 1,440 5 View

Is there a protein that binds DNA stoichiometrically and sequence-nonspecifically?

For some experiments, I need a control for DNA binding of proteins; and I need a protein that binds DNA sequence-nonspecifically, and, equally importantly, stoichiometrically. Does anyone know of...

01 January 2014 9,671 6 View

What is the nature of global gene expression changes upon serum-starvation in mammalian cultured cells?

There are plenty of reports dealing with serum-dependent/stimulated gene expression profiling in mammalian cells (for example: http://www.ncbi.nlm.nih.gov/pubmed/9872747). These studies usually...

09 September 2013 6,349 4 View