Anil Upreti

4 Questions 9 Answers 0 Followers

Questions related from Anil Upreti

Can any one suggest me the best protocol for RNA binding protein immuno-precipitation for mRNA of interest?

I want to identify the unknown RNA binding protein(RBP) in few mRNAs of interest. I am looking for a reliable immune-precipitation method (or any other possible method) to find the unknown RBP...

18 August 2020 1,883 4 View

Can the microscope (with filters not laser ) differentiate between GFP and cerulean during live cell imaging ?

I have cell line expressing cerulean and GFP at different time point. I am having difficulty identifying the exact fluorescent protein being expressed since the wavelength overlap for GFP and...

22 January 2020 5,438 6 View

What is the mechanism behind the gene activation using CRISPR?

How does the gene activation takes place by the use of VP64, VPR using the dead Cas 9 System?

05 November 2018 9,904 2 View

How can we identify the functional partner of protein?

How can we test if the protein requires help of other protein for function or works alone ?

10 September 2018 8,369 4 View