Anil Pooran

4 Questions 7 Answers 0 Followers

Questions related from Anil Pooran

How to distinguish between neutrophils and macrophages/monocytes in human BAL cells and whole blood samples by flow cytometry?

I am currently looking at specific immune markers in the lungs (BAL cells) and whole blood of human subjects by flow cytometry. We are trying to determine the expression levels of different cell...

28 August 2015 2,393 2 View

Effect of Glutathione S-transferase (GST) and Glutathione on T cell proliferation

I have expressed a human cytokine and two of its splice variants using the insect cell baculovirus system in sf21 cells. I have used a GST fusion protein for one of the mutants as it provided the...

23 July 2012 7,412 2 View

What should the cell viability be at the time of harvest in baculovirus protein expression system?

I am expressing a mutated human cytokine using the baculovirus system in shaker culture using sf21 cells. I have expressed the protein before which has shown up on a gel and western blot. However,...

23 May 2012 9,450 4 View

Qiagen rotor gene sybrgreen kit volume

I am doing some gene expression studies using the rotorgene 6000 (or rotorgene Q). I have recently bought the Qiagen rotorgene sybr green which I am using to do some optimization experiments. The...

07 February 2011 1,728 0 View