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Dear All, I am trying to differentiation of mono-mac 6 cell line by PMA treatment. for that i hav e treated cells with 20ng/mL of PMA for 24 hr. But, it does not differentiate to Macrophase...
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I have cloned a mammalian gene through the gateway cloning (Phase Lamda ) method. The codon optimization was done via IDT tools. The orientation of the insert has been confirmed by Sanger...
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Dear All, Is there any difference between Recombinant antigen preparation for ELISA AND Immuno Chromatographic Test. As we have found that recombinant antigens worked very well in ELISA...
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Dear All, Can we use two poly clonal antibodies generated against specific antigen in rabbit and mice simultaneously in the ELISA?
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Dear All, I am working on development of hybridoma technology. I was used balb/C mice for immunization and SpO cell lines as myeloma cells. After 3rd booster spleen was removed aseptically....
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