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I am using a pET28B vector to clone my 2.1kb insert digested with BamH1 (fwd) and Xho1 (rev) restriction enzymes. I'm using over 1ug of DNA for restriction digestion, kept at 37°C for over 3...
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While using the overlap PCR method for site directed mutagenesis, whenever I'm trying to anneal two fragments (A-1700bp, B-900bp), I'm getting a final product band at 900bp (i.e. only band B is...
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